Histology processing of a miniscule biopsy is a real challenge for surgical pathology. Some biopsies are so small that they are bordering cytology but these specimens have all histology organ/tissue features. For example, vocal cord biopsies often are like films of tissue.
Obviously, these specimens are very difficult for embedding and microtome cutting. The first trim takes the valuable material.
Application of many layers of dye, preferably Hematoxyline, on the specimen could be used to artificially enhance the size of the miniscule biopsy. The dye crystallizes on the surface coating the specimen. This crystal shield protects the main fragment while trimming. Hematoxyline over staining is not a problem for following slide staining.
This method was successfully tasted in many occasions. It is especially useful in brain biopsies with scarce material when a single almost invisible fragment solely represent diagnostically valuable structures.